Figure 4.

Loss of Dicer causes a range of spindle and chromosome congression defects. (A) Frequency distribution of Dicer^−/− oocyte phenotypes. The absolute number of oocytes observed with a given phenotype is indicated above the bar. One oocyte may fall into more than one phenotype class. (B) Quantification of number of spindles per oocyte in Dicer^−/− and wild-type oocytes. (C) β-Tubulin immunofluorescence staining of Dicer^−/− oocytes exhibiting several common phenotypes. DNA was counterstained with DAPI or Sytox green. (D) Centromere staining in Dicer^−/− oocytes. Oocytes were fixed in 3.7% paraformaldehyde and stained with a CREST antiserum together with a β-tubulin antibody; DNA was counterstained with DAPI.