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Time Series of YFP:ATK5 Localization during Mitotic Spindle Formation in an Arabidopsis Root Tip Cell.
(A) and (B) Prophase spindle. YFP:ATK5 localizes to MTs of the prophase spindle and PPB. Bar = 5 μm.
(C) to (E) Prophase spindle elongation.
(F) NEB.
(G) to (I) Prometaphase.
(J) to (L) Metaphase.
After NEB (F), YFP:ATK5 becomes enriched on interpolar MT bundles traversing the midzone (arrow in [H]). Intervals between frames are 1 min.
