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. 2007 Jan;19(1):196–210. doi: 10.1105/tpc.105.039594

Figure 2.

Figure 2.

Effects of emp4-1 on Aleurone and Basal Transfer Layer Development.

(A) to (D) Longitudinal section of 20-DAP wild-type ([A] and [C]) and mutant ([B] and [D]) kernels carrying the pVP1-GUS transgene and showing GUS precipitate in embryo and aleurone cells.

(E) to (H) Longitudinal section of 14-DAP wild-type ([E] and [G]) and emp4-1 ([F] and [H]) kernels carrying the pBET1-GUS transgene and showing GUS precipitate in basal endosperm transfer layer cells.

(C), (D), (G), and (H) are 10-μm wax sections stained with periodic acid Schiff's stain. Arrows and arrowheads highlight areas of interest. al, aleurone; betl, basal endosperm transfer layer; cse, central starchy endosperm; e, embryo; esr, embryo-surrounding region; nu, nucellus; p, placento-chalazal region. Bars = 2 mm in (A) and (B), 100 μm in (C) and (D), 1 mm in (E) and (F), and 200 μm in (G) and (H).