Figure 6.

emp4 Is Expressed in Vegetative and Reproductive Tissues.

RNA was isolated from leaves, roots, and stems (lanes 1 to 3), anthers (lane 4), ovaries (lane 5), 6-DAP embryos and endosperms (lanes 6 and 7), and 12-DAP embryos and endosperms (lanes 8 and 9). Quantitative RT-PCR analysis was performed using emp4 gene-specific primers, and Actin expression was used to normalize expression values (see Supplemental Table 1 online). Transcript abundance is indicated relative to 12-DAP endosperms, and error bars indicate se (see Methods).