Figure 9.

Expression of Genes Involved in Multiple Developmental Processes Was Altered in pip5k9-d and cinv1.

(A) Altered expression of multiple genes with enhanced PIP5K9 transcript levels or reduced CINV1 transcript levels. Seedling roots of pip5k9-d, cinv1, and wild-type plants were used for RNA extraction and further hybridization. Genes with significant differences in expression (Wilcoxon rank test, P < 0.01) were collected and functionally classified between wild-type and pip5k9-d plants (left panel) and between wild-type and cinv1 plants (right panel).

(B) Real-time RT-PCR analysis showed that RBCs was repressed in pip5k9-d and cinv1 seedlings (grown for 4 d). Transcript levels were normalized with respect to TUB4 transcript levels. Mean values were obtained from three independent PCR amplifications, and error bars indicate se. Heteroscedastic t test analysis showed significant differences (*P < 0.01).

(C) Summary of genes with altered transcript levels through microarray analysis, as well as changes in relevant metabolites and developmental processes. Open arrows indicate the regulatory tendency of the physiological process under enhanced PIP5K9, and the levels of relevant metabolites are highlighted (blue; decreased). The gene regulatory tendencies are highlighted in blue (downregulation) and red (upregulation).