Figure 4.
SP11 Binding Activities of the Membrane-Anchored Forms of SLG and SRK.
(A) Expression of the membrane-anchored forms of SLG8 (mSLG8) and SRK8 (mSRK8). Whole protein extracts (total), soluble fractions (sol), and microsomal membrane fractions (mm) of mSLG8- or mSRK8-expressing BY-2 cells were analyzed by immunoblotting with the antibody Ab-SLG8-C1.
(B) The microsomal fractions of mSLG8- and mSRK8-expressing BY-2 cells were incubated with biotin-S8-SP11 (+) or biotin (−) and pulled down using streptavidin–Sepharose. Interacting proteins (pull-down) and the proteins before precipitation (input) were analyzed by immunoblotting with the antibody Ab-SLG8-C1.