Figure 6.
SP11 Binding Activities of the Artificially Dimerized eSRK.
(A) The HLH-ZIP–fused extracellular domain of eSRK8 (eSRK8-HLH-ZIP) was incubated with biotin-S8-SP11 (+) or biotin (−). Interacting proteins (pull-down) and the proteins before precipitation (input) were analyzed by immunoblotting with the antibody Ab-SLG8-C1.
(B) The binding of eSRK9 and eSRK9-HLH-ZIP to biotin-S9-SP11 was analyzed as in (A).
(C) The stigmatic microsomal membrane fraction of S9-homozygotes was incubated with biotin-S9-SP11 in the presence of soluble eSRK9 or eSRK9-HLH-ZIP. Binding was analyzed as in (A).