Table 1.
Survey of A. thaliana Accessions That Produce Amplification Products with SRKA, SRKB, or SRKC Primers
| ΨSRKa
|
||||||||
|---|---|---|---|---|---|---|---|---|
| ABRC No. | Name | Geographical Origin | A | B | C | ΨSCR1b | ATREP3c | CCOPd |
| 1092 | Col-0 | Germany/U.S. | + | A1 (0.5 and 0.6 kb) | 3 kb | − | ||
| 20 | Ler-0 | + | A1* | 3 kb | − | |||
| 913 | RLD | Russia | + | A1 | 3 kb | − | ||
| 22605 | Tamm-27 | Finland | + | A1 | 3 kb | − | ||
| 22655 | Ms-0 | Moscow | + | A1 | 3 kb | − | ||
| 915 | Ws | Russia | + | A1* | 1 + 3 kb | − | ||
| 1150 | Est-1 | Russia | + | A1 | 3 kb | + | ||
| 1212 | Gu-1 | Germany | + | A1 | 3 kb | + | ||
| 1394 | No-0 | Germany | + | A1 | 3 kb | + | ||
| 1640 | Tsu | Japan | + | A1 | 3 kb | + | ||
| 6926 | Tsu1 | Japan | + | A1* | 3 kb | + | ||
| 22604 | Tamm-2 | Finland | + | A1 | 3 kb | + | ||
| 22606 | KZ1 | Kazakhstan | + | A1 | 3 kb | + | ||
| 22624 | Yo-0 | U.S. | + | A1 | 3 kb | + | ||
| 1124 | Ei-2 | Russia | + | A1 | 1 kb | − | ||
| 1390 | Nd-0 | Germany | + | A1 | 1 kb | − | ||
| 3109 | Ber | Denmark | + | A1 | 1 kb | − | ||
| 22659 | Ws-2 | Ukraine | + | A1 | 1 kb | − | ||
| 1602 | Ws-0 | Ukraine | + | A1 | 1 kb | + | ||
| 22567 | Knox-18 | U.S. | + | A1 | 1 kb | + | ||
| 22584 | Omo2-1 | Sweden | + | A1 | 1 + 2 kb | + | ||
| 22574 | Lov-1 | N. Sweden | + | A2 (0.5 and 0.9 kb) | 1 kb | + | ||
| 22575 | Lov-5 | N. Sweden | + | A2 | 1 kb | + | ||
| 22643 | Nok-3 | Netherlands | + | A2 | 1 kb | + | ||
| 22576 | Fab-2 | Sweden | + | A2 | 1 kb | − | ||
| 22579 | Bil-7 | Sweden | + | A2 | 1 kb | − | ||
| 22581 | Var2-6 | Sweden | + | A2 | 1 kb | − | ||
| 22583 | Spr1-6 | Sweden | + | A2 | 1 kb | − | ||
| 22586 | Ull2-5 | Sweden | + | A2 | 1 kb | − | ||
| 22580 | Var2-1 | Sweden | + | A2 | − | + | ||
| 1500 | Sah-0 | Spain | + | A3 (1 kb) | 1 kb | − | ||
| 954 | Bay-0 | Germany | + | A4 (−) | 1 kb | + | ||
| 6179 | Hodja | Tajikistan | + | A4 | 1 kb | − | ||
| 1096 | Cvi-0 | Cape Verde Islands | + | −* | 1 kb | + | ||
| 1264 | Kas-2 | India | + | −* | 1 kb | + | ||
| 1354 | Lz-0 | France | + | − | 1 kb | + | ||
| 22628 | Br-0 | Czechoslovakia | + | − | 1 kb | + | ||
| 22649 | Pro-0 | Spain | + | ND | 1 kb | + | ||
| 1244 | Ita-0 | Morocco | + | −* | 3 kb | + | ||
| 22632 | Ra-0 | France | + | − | 3 kb | + | ||
| 1372/6795 | Mr-0 | Italy | + | − | − | + | ||
| 22656 | Bur-0 | Ireland | + | − | − | + | ||
PCR using primers for ΨSRKA, ΨSRKB, and ΨSRKC. +, successful amplification; blank boxes, no amplification.
DNA gel blot analysis with the ΨSCR1 probe. A1, A2, and A3: three different ΨSCR1 hybridization patterns (sizes of hybridizing HincII fragments are indicated in parentheses). −, no hybridization signal; ND, not determined. Asterisks indicate accessions that were reported to contain ΨSCR1 by Shimizu et al. (2004).
PCR using primers (see Supplemental Table 1 online) specific for the ATREP3 insertion/deletion (Figure 1A). The sizes of amplification products are shown. −, no amplification.
PCR using primers (see Supplemental Table 1 online) specific for CCOP, a 675-bp product from the region between ΨSRKC and COPIA53 in C24 (Figure 1B). +, successful amplification; −, no amplification.