Figure 5.

CRY2-GFP mRNA Expression in Mesophyll and Vascular Bundles.

Mesophyll protoplasts (top panel) and vascular bundles (bottom panel) were isolated from cotyledons in the seedlings grown for 10 d under LD. The samples were analyzed by quantitative RT-PCR. The averages of three biological replicates are shown. TUB2/TUB3 was used as an internal control for calculating relative levels of CRY2-GFP mRNA in C2G lines and CRY2 mRNA in the wild type. Data were normalized to the level of CRY2 mRNA in vascular bundles of the wild type, which was set to 1 arbitrary unit (a.u.). Mean ± se (n = 3). The lines shown are pCRY-C2G-16, pCAB-C2G-6, pSUC-C2G-2, pSultr-C2G-10, pML-C2G-6, pCER-C2G-8, pUFO-C2G-13, and pAt3g-C2G-7.