Figure 9.

GA Levels in Developing Siliques of gamt Single and Double Mutants.

Shown are endogenous levels of GAs in developing siliques of the wild type, gamt1-1 and gamt2-2 single mutants, and the gamt1-2 gamt2-2 double mutant plants. Results of respective GAs are displayed according to their order in the biosynthesis pathway (see Figure 1). Results of GA₄ are also indicated in the inset with a different y axis scale to clarify the differences among the genotypes. Note that the y axis scale for precursor GAs (GA₁₂, GA₁₅, GA₂₄, GA₉, GA₅₃, GA₄₄, GA₁₉, and GA₂₀) is different from that for bioactive (GA₄ and GA₁) and deactivated (GA₃₄ and GA₈) forms. GA measurements were conducted three times using independently prepared plant extracts. Means with se (which are displayed only when they are >0.2) from triplicates are shown. Asterisks indicate that these GAs could be measured reliably only once due to comigration of impurities on the liquid chromatograph. d.w., dry weight.