Table 3.
Endogenous GA Levels in Arabidopsis Plants Overexpressing GAMT1 or GAMT2
| Wild-Type Control for GAMT1-OEa | GAMT1-OE Line 13-5a | GAMT1-OE Line 13-6a | Wild-Type Control for GAMT2-OEb | GAMT2-OE Line 12-3b | GAMT2-OE Line 12-5b | ||
|---|---|---|---|---|---|---|---|
| 13-H | GA12 | 15.4/13.2 | 0.7/4.1 | 4.3/1.2 | 21.5/14.0 | 16.9/10.2 | 10.7/15.0 |
| GAs | GA15 | 1.5/3.2 | 0.2/1.3 | 0.4/1.2 | 4.4/1.7 | 10.8/7.8 | 11.8/10.6 |
| GA24 | –/21.7 | –/2.9 | –/2.9 | 24.6/16.9 | 14.6/10.8 | 10.7/15.2 | |
| GA9 | 0.4/ND | 0.1/ND | 0.1/ND | ND/ND | ND/ND | ND/ND | |
| GA4 | 0.8/2.3 | 0.1/0.1 | ND/0.1 | 0.9/0.4 | 0.1/0.1 | 0.2/0.2 | |
| 13-OH | GA53 | 2.4/2.4 | 0.6/0.4 | 0.1/0.3 | 2.8/1.6 | 1.4/1.1 | 0.6/1.0 |
| GAs | GA44 | ND/ND | ND/ND | ND/ND | ND/ND | ND/ND | ND/ND |
| GA19 | –/16 | –/3.6 | –/2.9 | 8.2/3.0 | 9.5/6.3 | 8.2/10.5 | |
| GA20 | 0.4/0.4 | 0.1/ND | 0.1/ND | 0.1/ND | 0.1/ND | 0.1/0.1 | |
| GA1 | 0.2/0.5 | ND/ND | ND/ND | ND/ND | ND/ND | ND/ND |
GA measurements were performed twice using independently prepared plant materials. Values (ng/g dry weight) from both the first (left) and second (right) measurements are shown. –, Detectable, but could not be quantified reliably due to comigration of impurities. ND, not detected or could not be quantified reliably due to low abundance.
a,b
These two sets of plant materials were grown on different occasions. OE, overexpressing.