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. 2007 Mar 5;7:40. doi: 10.1186/1471-2407-7-40

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Copyright © 2007 Jiang et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Alteration of peripheral lymphocyte subsets and NK cells with tumor progression. T lymphocyte subpopulations were quantitated by flow cytometry. Samples were prepared by adding 5 μl of fluorescent monoclonal antibody of CD3, CD4, CD8 and CD161a to 100 μl of heparinized whole blood. CD3+CD4+ (T helper cell) and CD3+CD8+ (T suppressor cells) were measured and natural killer (NK) was recognized as CD3-CD161a+. Values (except for CD4+/CD8+) represent percentage (%). Data are expressed as averages and SD. * means compared with control group, P < 0.05. # means compared with group A, P < 0.05. $ means compared with group B, P < 0.05.