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. 2007 Mar 22;117(4):889–901. doi: 10.1172/JCI30556

Figure 2. NF-κB activity is deregulated during postnatal development of mdx mice.

Figure 2

(A) Muscle extracts from 5-week-old WT;3xκB-Luc-Tg or mdx;3xκB-Luc-Tg mice were prepared for luciferase assays. Luciferase values were normalized to total protein. *P < 0.05; n = 5. (B) Gastrocnemius muscles were immunostained for luciferase expression in 7-week-old WT;3xκB-Luc-Tg and mdx;3xκB-Luc-Tg mice (n = 3). Scale bar: 50 μm. (C) EMSAs for NF-κB and Oct-1 performed in gastrocnemius muscles from WT and mdx mice during postnatal development. (D) Primary myoblasts isolated from 6-day-old WT;3xκ-Luc-Tg and mdx;3xκB-Luc-Tg mice were differentiated and subsequently switched to either medium alone or medium containing TNF-α (5 ng/ml) for 6 hours.