Figure 3.

Adenoviral-mediated expression of the C114R PPARγ mutant inhibits human preadipocyte differentiation

Chub-S7 human preadipocyte cells were infected with comparable efficiency using recombinant adenoviruses expressing GFP, GFP-WT, or GFP-C114R mutant PPARγ and differentiated in the presence of rosiglitazone (100nM).

A) Fully differentiated Chub-S7 cells fixed and stained with oil red O.

B) aP2 expression quantitated by real-time qPCR at days 0, 3, 5, and 7 postdifferentiation with results representing the mean ± SEM of at least three independent experiments in triplicate.

C) Western blotting of Chub-S7 cells at day 4 posttransduction with recombinant adenoviruses confirming comparable levels of total receptor expression. Nondifferentiated, (day 0) nontransduced, cell extracts (ND) are shown for comparison. The positions of PPARγ (open arrow) and nonspecific band (solid arrowhead) are indicated.