Figure 3. .

Skeleton analyses. Side and top views of skeleton preparations of a wild-type mouse (A and B), a Df(11)17/+ mouse (C and D), a Df(11)17-1/+ mouse (E and F), and a Rai1^−/+ mouse (G and H) are presented. I, Measurement results for Df(11)17/+ mice (DEL n=17, WT n=28), Df(11)17-1/+ mice (DEL n=18, WT n=13), and Rai1 ^−/+ mice (DEL n=19, WT n=12). Mean ± SEM values are presented. Landmarks used for the measurements are labeled (A and B): a and c = anterior notches on frontal processes situated laterally in relation to the infraorbital fissure, b = nasale, d = intersection of parietal and interparietal bones, e = intersection of interparietal and occipital bones at the midline, f = bregma, and g = intersection of zygoma with zygomatic process of temporal superior aspect.⁴³ WT = wild type; asterisk indicates P<.05. Skeleton preparations were performed as follows. Mice were sacrificed, skinned, eviscerated, and dehydrated in 95% ethanol (EtOH). After the staining of cartilage with 0.05% alcian blue 8GX solution, mice were rinsed in 95% EtOH and were cleared in 2% KOH. Alizarin red (0.015% in 1% KOH) was then used to stain the bones, followed by clearing in 1% KOH and 20% glycerol. Images were obtained with Image Pro Plus software (Media Cybernetics) and were analyzed using Adobe Photoshop version 5.5 (Adobe Systems).