Figure 6. .

a, Full-length cloning of a TAF1 isoform sequence containing alternative exon 34′. The 5′ end was obtained from CapSite cDNA from brain. The 3′ end was obtained from Marathon-Ready cDNA from brain. The complete DNA sequences of these long products were determined by a PCR direct-sequencing method by use of 28 redundant internal sequencing primers (red triangles). b, From the long PCR products, products of sufficient quantity and quality for PCR direct sequencing were amplified. NC = negative control without DNA template. c, Each exon 34′–specific primer was designed to have only 3 nt of exon 34′, to prevent erroneous amplification due to slippage.