Table 3. .

PCR Primer Sets for Fragment Amplification from the TAF1 cDNAs^[Note]

	Forward Primer			Reverse Primer
Fragment Name	Namea	Sequence (5′→3′)	Tmb	Namea	Sequence (5′→3′)	Tmb	Amplicon Size (bp)
TA01	TAF_f_3	GGGAGCTCAGTAFAGTCACTTCTG	60.6	TAF_r_400	CCACCTCATTGATGTCTGAATAG	59.6	398
TA02	TAF_f_377	ACTAFTTCAGACATCAATGAGGTGG	60.4	TAF_r_772	TGGCATCATGCTGCATAATC	61.8	396
TA03	TAF_f_755	TTAFTGCAGCATGATGCCAC	60.4	TAF_r_1175	CCCAGCATATCATACCACAGTC	60.4	421
TA04	TAF_f_1152	CCGACTGTGGTAFTGATAFTGCTG	61.5	TAF_r_1550	CGTCCATATACCAGATCCTCATTG	62.7	399
TA05	TAF_f_1528	AATGAGGATCTGGTAFTAFTGGACG	60.2	TAF_r_1910	CGTAATTCCACAGCAGGAATTG	62.7	383
TA06	TAF_f_1889	CAATTCCTGCTGTGGAATTAFCG	62.7	TAF_r_2273	CCATATTTACAATCTGGTGCTCC	60.7	385
TA07	TAF_f_2251	GGAGCACCAGATTGTAFAATAFTGG	60.7	TAF_r_2598	TTCCATTCGTATCCTCCGTG	62	348
TA08	TAF_f_2580	ACGGAGGATAFCGAATGGAAG	60.1	TAF_r_2979	ATCTGCCACCCCAGTCAC	60.8	400
TA09	TAF_f_2945	GCAAGTGTCTGCTAFGAGGTGAC	60.3	TAF_r_3331	GTAGGTCAAAGATGCGCTGAC	61	387
TA10	TAF_f_3311	GTCAGCGCATCTTTGACCTAFC	61	TAF_r_3710	GTCCGTATGCGCACATAGG	61.3	400
TA11	TAF_f_3689	ATGCCTAFTGTGCGCATAFCG	61.8	TAF_r_4089	CAAGACAATTTTGGTCCCTTC	59.6	401
TA12	TAF_f_4069	GAAGGGACCAAAATTGTCTTG	59.6	TAF_r_4467	TAGCTCCAGATGCTCTCTGAAC	59.9	399
TA13	TAF_f_4413	CGTGCGTAFAACGCCTCTAFC	60.6	TAF_r_4808	CGACTCTGATACTTGTGCTTGG	61	396
TA14	TAF_f_4780	AACATCTCCAAGCACAAGTAFTCAG	60.7	TAF_r_5164	GCTTTTCTGGAGTGGCACTG	62.1	385
TA15	TAF_f_5130	TGTCTTGGATAFTTCCCAGTGC	61.1	TAF_r_5505	GTTGCTATCCTCCAAACCATG	60.5	376
TA16	TAF_f_5482	TCCCATGGTTTGGAGGATAFG	60.9	TAF_r_5862	AAGGCTAAGGTGTTAGTTAATTTCATG	60.3	381
TA17	TAF_f_5834	ATCATGAAATTAFACTAFACACCTTAFGCC	60.1	TAF_r_6231	TTAGTAGAGATGCGGTTTCGC	60.5	398
TA18	TAF_f_6073	GTAFACTTTAFTGTCCTCTTGATGTAFTTAFGG	59.1	TAF_r_6469	CAGGAGGGCTCTCATCTGC	62.3	397
TA19	TAF_f_6451	GCAGATGAGAGCCCTCCTG	62.3	TAF_r_6855	AAGGATCTGATAGAGTGCTTATCATG	60.2	405
TA20	TAF_f_6831	ATGATAFAGCACTCTAFTCAGATCCTTG	60.2	TAF_r_7164	CACACTCTGCCATTTCTAGACTG	60.1	334
TA21	TAF_f_7140	CACAGTCTAFGAAATGGCAGAGTG	60.1	TAF_r_7553	GGGTTATCATTGTGAACAGTTAGC	59.9	414
TA22	TAF_f_7277	GGCAGGAAGTAFTAFTCATCACAAGC	62.1	TAF_r_7606	CCAGCATACATAACAAACACAGAAG	60.9	330
MT23c	TAF_f_7277	GGCAGGAAGTAFTAFTCATCACAAGC	62.1	pMTS-r	GTGAGAGCTCAAAGACCAATAAG	58.3	779
Probe 1	TA_f_2333	TGCAAGCATTTGAGAACAACCT	62	TA_r_2690	GAGTCCATCCCTGTGCGTT	61.1	358
Probe 2	TA_f_4786	TCCAAGCACAAGTATCAGAGTCG	61.7	TA_r_5185	CTTCACCTTCCTGTGTTACCTGCT	63	400
Probe 3	TA_f_5637	GAGCGTACTAAGCCAGGTCCA	61.7	TA_r_6065	TTCATAATTTCCCCTCCTTCCC	62.4	393

Note— The PCR mixture contained 2 μl of the first-strand cDNA, 0.2 mM of each dNTP, 1 μM of each primer, 1× GeneAmp PCR buffer, and 2.5 units of AmpliTaq Gold DNA polymerase, in a 50-μl total volume. The PCR conditions were 9 min at 95°C, followed by 35 cycles at 95°C for 45 s, 60°C for 45 s, and 72°C for 60 s.

^aThe value in the third part of the primer name represents position in a major form of the TAF1 transcript.

^bT_m = annealing temperature (°C). The calculation conditions were 1,000 nM of each primer and 50 mM potassium ions.

^cThe primer set was used only for first-strand cDNA from MTS.