Table 4. .

Primers and Probes for the TaqMan Expression Assay

	Primer Sequence(5′→3′)
Assay Name	Forward	Reverse	Reporter 1 Sequencea (5′→3′)	Amplicon Size (bp)
TAF1-5′	GACTGACGGTGCCTTGGT	GTCTGAATAGTCCACAGCATCTTCT	ACCCACCCTTCATCATTT	70
TAF1-3′	ACCTTATTCTGGCCAACAGTGTT	ACAATCTCCTGGGCAGTCTTAGTAT	ACTCTCAGGTCCATTATAC	73
TA02-334	TGGGACCAATGAAGAAGGATAAGGA	ATTCTGAGTCAGAGGAACTACTGAAGT	CCACTTTCTCACCAGTAATAG	81
TA08-269	CTCTGCGCTGACTTCAAACG	TTCCTCATTTTCTTCTTCTGGAGCAA	CCATAGCCAGCATCCTGT	79
TA09-391	GTGGTGAAGGATTCTCCTATGTGAA	ATCTGTTCCTGTCACTGTCTTCTTC	CAGCAGAAGGTAGGATGATAA	105
TA09-693	TGCCAAGCAACTTCTACGTAAATTTG	TCCAACTCTATACAAAGCCCCTAGT	CACACTCACCCTCTTCC	99
TA14-317	ACCTTATTCTGGCCAACAGTGTT	CCTCCAAAGCTGCTTCTTTAGCA	TTGAGTCAAATGTTCATCATACATT	97
TA14-389	GGAGATTGTGAACGTCTGTTACCA	TCCTTCTCAAGTTGAGTCAAATGTTCAT	CATACTACCTCAGTCAATGTC	73
TA14-391	CCCCAGGGCCCTACAC	CTGAGGGATGTGTTGGTATCATACAA	CCTCAGGCTAAGCCTC	64
TA14-407	GGGAGAGCTTTCTGGATGATGTAAA	ACAATCTCCTGGGCAGTCTTAGTAT	CTGACTCTCAGGTCCTGCACG	119
TA15-477	AGTGCCACTCCAGAAAAGCA	CCTCGCCCAGCCTACCT	CCTGGCGCATCTGTGT	61
TA18-261	GTGGCTCACACCTGTAATCTCA	CCTCCCGGGTTCAAGTAATTCTC	CAGCCTCCCAGAGTGC	67
TA14-385Nb	CCCCAGGGCCCTACAC	CTGAGGGATGTGTTGGTATCATACAA	CCTCAGCCTCCTGATT	58
TAF1-M	GCTAAAGCTCTGCGCTGACT	TTAAGCACCCACCAGTTTGAGT	CATCCCTGTGCGTTTGA	60
TAF1-3′N	CCCAGTGCCACTCCAGAAAA	CAGTTCCTTCCTCTTCATCTGCAA	ACCTTCCTGTGTTACCTGC	82
MTS-V4	TTGCTCTTGGGCTCTGCATT	CTGGCACGGATTTTCACTATCTT	ACTCCTTACAGGTACCAATGA	251
MTS-37/1	CCCATGGTTTGGAGGATAGCA	CATCTCTGAATGGCTTGTTCATTG	TCAGGTGATGAACTTCAATA	166
MTS-37/3	CCCATGGTTTGGAGGATAGCA	TCGTTGCTCCAGAGATCTTTGTG	ACATCAGGTACCAATGAAC	83
MTS-2/3	GGCCTCCGGCATTGCT	TCGTTGCTCCAGAGATCTTTGTG	TCCACTGTACCAATGAA	112
MTS-32′/34′	CAACAGTGTTAAGTATAATGGGTACATGTG	CTGAGGGATGTGTTGGTATCATACAA	TCAGGCTAAGCCTCCT	268
MTS-3/4	GTGTCCGGAGTTGGTTCCT	TGGCACGGATTTTCACTATCTTCA	CCTTCGCGCTTCAGGC	105

^aFor the reporter 1 sequences, the dye was FAM.

^bThis probe is designed to detect all isoform sequences not containing exon 34′.