Skip to main content
. 2000 Mar 28;97(8):4239–4244. doi: 10.1073/pnas.070371497

Figure 3.

Figure 3

Gene expression in ventricles from Nppb+/+ and Nppb−/− mice. (A) Northern blot analysis of Acta1, Atp2a2, Nppa, Edn1, and Tgfb1 mRNAs in ventricles from Nppb+/+ mice (n = 10) and Nppb−/− mice with or without cardiac fibrosis (n = 5 for each) at 20 wk of age. Hybridization signal for Gapd mRNA was used as an internal control. Total RNAs (20 μg) were analyzed, and representative cases are presented. (B–F) Shown are RT-PCR and Southern blot analysis of Ace mRNA (B and C) and Northern blot analysis of Tgfb3 and Cola1 mRNAs (D–F) in ventricles from Nppb+/+ and Nppb−/− mice at 10 wk of age (n = 5 for each) and ventricles from Nppb+/+ mice (n = 15) and Nppb−/− mice with or without cardiac fibrosis (n = 8 for each) at 20 wk of age. Representative cases (B and D) and quantification of Ace (C), Tgfb3 (E), and Cola1 (F) mRNA levels are shown. Open, hatched, and filled bars represent mRNA levels for Nppb+/+ mice, Nppb−/− mice without cardiac fibrosis, and Nppb−/− mice with cardiac fibrosis, respectively. The mean mRNA levels in Nppb+/+ mice (open bars) were defined as 1.0 arbitrary unit. ∗, P < 0.05; ∗∗, P < 0.01 vs. Nppb+/+ mice.