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Hypocretin accumulates postnatally specifically in the brain. Northern blot, prepared by standard methods with poly(A)+ from the indicated tissues of (A) adult rats or (B) whole brains (E14 was whole head) from developing rats of the indicated embryonic (E) or postnatal (P) ages. In each case the blot was stripped and reprobed with a cDNA for the ubiquitously expressed cyclophilin as a loading and RNA integrity control.
