Table 2.

PCR conditions. PCR reactions were performed with DynaEXT with standard protocols; exon 6 and 7 were amplified as one fragment.

	Exons 2, 3, 4, 5, 9, 10	Exon 1	Exons 6–7,8
DNA	25 ng	25 ng	25 ng
10×Buffer w.MgCl2	2,5 μl	2,5 μl	2,5 μl
10 mM dNTPs	0,5 μl	1 μl	0,5 μl
10 μM forward primer	1 μl	1 μl	1 μl
10 μM reverse primer	1 μl	1 μl	1 μl
DyNAzyme™ EXT	0,5 U	1 U	0,75 U
DMSO	0	1 μl	0
H2O	To 25 μl	To 25 μl	To 25 μl