Figure 1.

RT-PCR/PAGE assay. (a) Analysis of representative ethidium bromide-stained polyacrylamide gels. Bands at 150 bp are the PAW 108 internal control PCR product. Every PCR band is from a different animal. Time series of normalized ratiometric densitometry data (averaged ± SEM) are graphed to the right of each gel. (b) Dynamic range of RT-PCR assay. The relationship between the log(starting molecules) and the log(product/control) ratio is linear between 10 and 10⁸ molecules for GAD65 (squares) and GAD67 (triangles; for details, see ref. 4). (c) Range of ratiometric values. The histogram shows the distribution of densitometrically determined product ratios. The measurement values (c) are comfortably within the linear range of the log–log assay (b), far removed from potential saturation.