Figure 3.

Inhibition of DNFB specific T cell proliferation by MV infection. To induce the generation of DNFB-specific T cells, one ear of cotton rats was painted with 40 μl of a 1% DNFB solution on 3 consecutive days. Mock-infected and cotton rats infected 3 days before sensitization were used. On day 4, the draining lymph nodes from both ears were sampled and single cell suspensions were produced. Triplicates of 5 × 10⁵ lymphocytes per well in a 96-well plate were labeled with 0.5 μCi of [³H]thymidine per well and harvested 20 h later to measure proliferation. The data are given as an average of four animals per group.