Figure 1.

Soluble components present within human LC selectively enhance CD1d expression on the surface of IECs. (a) Cell surface ELISA of T84 monolayers following incubation with either 6 μg/ml (black bars) or 3 μg/ml (gray bars) of LC sample for varying periods of time. (b) Relative levels of CD1d, MHC class II, and ICAM-1 expression were compared by ELISA on T84 cells incubated alone (CNT) or in the presence of 6 μg/ml LC sample (LC) for a period of 48 hours. As a positive control for the detection of all inducible epithelial markers, monolayers were exposed to IFN-γ for 48 hours at a final concentration of 1,000 IU/ml. ODs at 405 nm ± SEM, generated by ELISA, were blanked against isotype-matched control antibodies.