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Generation of the Ad-Yas virus carrying the HMGI(Y) gene in an antisense orientation. Homologous recombination between shuttle vector pac-CMVpLpA containing an expression cassette of mouse HMGI-Y cDNA in an antisense orientation (HMGI-Yas) (Left) and the adenovirus genome cloned in plasmid pJM17 (Right) generated an adenovirus clone in which the adenovirus E1A region was replaced by the HMGI(Y) cDNA expression cassette.
