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. 2000 Apr 4;97(8):4256–4261. doi: 10.1073/pnas.070029997

Figure 2.

Figure 2

Expression of the HMGI(Y) antisense sequences and inhibition of HMGI(Y) protein synthesis in human thyroid carcinoma cell lines by Ad-Yas. (A) Strand-specific RT-PCR assay for the HMGI(Y) antisense sequences in the PC Cl 3, HTC 2, ARO, and FB-1 cells infected with Ad-Yas (10 pfu per cell) and Ad-lacZ (10 pfu per cell). No amplification was obtained when ARO and FB-1 extracted from Ad-Yas-infected cells were analyzed without reverse transcriptase. (B) Western blot analysis of the HMGI(Y) proteins in ARO (Left) and FB-1 (Right) cells infected with Ad-Yas at different concentrations, as indicated, and Ad-lacZ. The PC MPSV cells are PC Cl 3 cells infected with the MPSV carrying the v-mos oncogene (20). These cells were used as a positive control. Analysis of γ-tubulin protein was performed as a control for protein loading.