Figure 7.
(A) Detection of the Ad-Yas virus in the ARO xenograft tumor by RT-PCR analysis after i.p. treatment. Strand-specific RT-PCR assay for the HMGI(Y) antisense sequences. The cDNA was coamplified with glyceraldehyde-3-phosphate dehydrogenase-specific primers as an internal control. No bands were detected in non-reverse-transcribed RNAs, thereby excluding DNA contamination. (B) Immunohistochemical detection of the Ad-Yas virus in the xenograft tumor after i.p. treatment. Paraffin sections from tumors treated with Ad-Yas were analyzed by immunohistochemistry after 48 h by using antibodies raised against the hexon from adenovirus type 2. No immunoreactivity was observed in untreated tumors (×40). Conversely, strong immunoreactivity was observed after i.p. treatment with either 5 × 107 pfu (×40) or 5 × 108 pfu (×40).