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BH4 levels in GCH-Tg and WT mouse tissues. Organs and plasma were harvested, and BH4 levels were determined by iodine oxidation in acid and base followed by HPLC. BH4 levels were increased threefold in GCH-Tg lung, heart, and aorta as compared with WT (*P < 0.05, n = 3–4) but not in liver or plasma.
