Figure 1.

LT toxicity in inbred mice. (a and b) Comparison of four doses of PA plus LF in BALB/cJ (a) and C57BL/6J (b) mice. Mice were injected i.p. with 1 ml of toxin in PBS. Results are based on n = 12, n = 24, n = 60, and n = 12 for 10 μg, 50 μg, 100 μg, and 250 μg of each toxin component. (c) Comparison of i.p. and i.v. routes of injection in the BALB/cJ mouse. Results are based on n = 18, n = 24, n = 60, and n = 20 for 50 μg i.v., 50 μg i.p., 100 μg i.p., and 100 μg i.v. of each toxin component. The differences in i.p. and i.v. survival curves at both 50 and 100 μg are not statistically significant by the log-rank test (P = 0.0807 and 0.553, respectively). The difference between the 50-μg i.p. curve and both 100-μg curves is statistically significant (P = 0.0013 and P = 0.0002). (d) Data from a and b for the 100-μg dose injected i.p., presented to allow direct comparison of the single selected dose used in all further experiments.