Figure 2.

LT effects on circulating monocytes, neutrophils, and platelets. Graphs show circulating monocyte (a), neutrophil (b), and platelet (c) levels in response to LT treatment as a percentage of untreated controls. Values are the mean of the percentage change in two to eight individual pooled experiments using both i.p. and i.v. treated mice at doses of 50 μg, 100 μg, and 250 μg LT, based on groups of two to four mice per time point. Values were calculated as the percentage change in cell number relative to a PBS-treated control group (two to four mice per experiment). Absolute control cell numbers differed in each experiment. Error bars indicate SD for the mean percentage change. Unpaired t test analysis indicates that differences in curves in a are statistically significant at 18, 24, and 36 hours (P = 0.008, P < 0.0001, and P < 0.0001, respectively). In b, differences in the two strains are only significant at 10 and 12 hours (P = 0.002 and P = 0.004, respectively). In c, differences in the two strains are not significant at any point. In a, differences relative to 0 hours are statistically significant at all times past 6 hours in BALB/cJ and at 6, 10, 12, and 36 hours in C57BL/6J. In b and c, differences relative to 0 hours are statistically significant beyond 10 hours in BALB/cJ and beyond 24 hours in C57BL/6J.