Figure 3.

Lymphocyte infiltrates are detected in the spinal cords of day-13 EAE rats in both groups; however, the positioning of infiltrates in LTβR-Ig–treated rats is altered. FACS analysis of lymphocytes obtained from the spinal cords is shown in a. The percentage of CD4⁺ (b) and CD8⁺ (c) for all cells in the lymphoid size gate was compared between treatment groups, where control rats were naive and untreated. Mean and standard deviation of five separate animals per group is shown. Spinal cords were also harvested, fixed, tangential paraffin sections stained with H&E, and visualized at ×50 (d, f, h) or ×400 (e, g, i) with a Leica microscope. Unimmunized naive rats (d and e) were compared with control huIgG–treated rats (f and g) or LTβR-Ig–treated rats (h and i). The experiments were performed twice with similar results.