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Modifying the human κ receptor to make the Ro1, E/Q, and Ro2 receptors. The wild-type sequence is shown as ○; residues that have been changed are shown as •. The EL2 loop exchange is included in Ro1 and Ro2 constructs. The E297Q mutation is included in the E/Q and Ro2 constructs. The FLAG epitope is included in all the constructs in this study.
