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Agonist activities of selected ligands on κ-WT, Ro1, and Ro2. Agonist-mediated changes in intracellular calcium were measured by the FLIPR as described in Materials and Methods. (A) Dose responses on κ-WT, Ro1, and Ro2. (B) Sample tracings of the actual calcium fluorescence curves from the 1 μM dose. The arrow indicates the addition of agonist.
