Figure 1.

Neutrophil accumulation in murine lungs after reperfusion. Mouse lungs were collected and homogenized for MPO determination after limb clamping with increased intervals of reperfusion (0, 3, and 24 h). Neutrophil numbers were obtained by using a calibration curve for MPO values. Neutrophils in lung biopsies from control mice (that received only pentobarbital, 50 mg/kg, i.p., n = 13 mice) and mice that underwent 3 h of limb clamping then continued with 0 (n = 13 mice), 3 (n = 16 mice), or 24 (n = 13 mice) h of reperfusion are indicated. Results are mean ± SEM of 13 or 16 mice. Asterisks indicate that the differences between 0 and 3 h of reperfusion as well as between 0 and 24 h of reperfusion are significant (P < 0.01). N.S., not significant. (Inset) Hematoxylin/eosin-stained lung biopsy from control mouse lung (Left) and lungs at 3 h clamping and 3 h reperfusion (Right). Arrowheads point to neutrophils within lung tissue. (×400.)