Far-UV CD spectra of host–guest peptides. CPOred is used to indicate the peptide with a Cys-Pro-Hyp guest triplet under reducing conditions. (A) Spectra are shown for peptides GPS (—), SPO (----), CPOred (-⋅⋅-⋅⋅), DPO (⋅⋅⋅⋅⋅⋅⋅⋅), and RPO (-⋅-⋅-) recorded at 0°C in PBS (concentration = 1 mg/ml). Corresponding spectra are shown for peptides GSO (B) and APO (C) recorded at TMAO concentrations of (from bottom to top at 225 nm) 0, 0.5, 1.0, 1.5, 2.0, and 3.7 M. (Insets) The increase of the molar ellipticity at 225 nm observed on increasing the concentrations of TMAO.