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Thermal denaturation of host–guest peptides at varying concentrations of TMAO. The change of ellipticity on heating was monitored at 225 nm for peptides GSO (A) and APO (B) (concentration = 1 mg/ml) in PBS (—) and after addition of 0.5 (----), 1.0 (⋅⋅⋅⋅⋅⋅⋅⋅), 2.0 (-⋅-⋅-⋅), and (for APO) 3.7 (-⋅⋅-⋅⋅) M TMAO. (Insets) The increase in melting temperature on increasing TMAO concentrations.
