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. 1998 Jan 6;95(1):376–381. doi: 10.1073/pnas.95.1.376

Figure 2.

Figure 2

RNA gel blot analysis of reporter line 16/56 retransformed with pLh-G4 and pL-VP16 activator constructs. RNA was isolated from reporter line 16/56 (R-16/56) containing pOp-GUS alone and from several lines retransformed with the activator plasmids pLh-G4 (G3 to G22) and pL-VP16 (V2–V11) that express LhG4 and L-VP16, respectively. Samples were taken from plants that had been maintained for several months in sterile culture by repeated stem cutting. The blot was probed initially for GUS transcripts, stripped, and reprobed with a fragment of the lacI gene that recognizes transcripts of both LhG4 and L-VP16.