Figure 4.

Activation of GUS reporter gene expression in the progeny of crosses between activator and reporter plants. Three activator lines transformed with pLh-G4 were each crossed with two reporter lines transformed with pOp-GUS and a control line transformed with plasmid pTA-GUS (Fig. 1C). Seeds were germinated in the presence of both hygromycin and methotrexate and the seedlings were stained for GUS activity. A single-stained seedling from each reporter line and each cross is shown. Reporter 1 = 16/56; Reporter 2 = 16/12. Activator 1 = line 10/1–6; Activator 2 = line 47/1–21; Activator 3 = line 10/1–2.