Figure 4.

Tissue-specific expression of ARC1. Total RNA from (A) root, stem, leaf, petal, anther, and pistil or (B) pistil, stigma, style, and ovary were hybridized to the ARC1 cDNA. The numbers 1 to 3 represent different bud sizes with 1 = 1–2-mm buds, 2 = 3–4-mm buds, and 3 = 5–7-mm buds. ARC1 transcripts are only detected in the pistil, and within the pistil, the ARC1 mRNA is restricted to the stigma. Hybridization of the filters with the thioredoxin-h clone, THL-1 (22), indicated that intact mRNA was present in all lanes (not shown). The filters were also hybridized with the 18S cDNA probe to show the relative amount of total RNA present in each lane.