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(A) Time course of p21 induction after the addition of 50 μM IPTG. p21 levels (in arbitrary units) were determined by ELISA by using WAF1 ELISA kit (Oncogene Science). (B) Time course of changes in [3H]thymidine labeling index (determined by autoradiography) after the addition of 50 μM IPTG. (C) Time course of changes in mitotic index (determined microscopically after 4′,6-diamidino-2-phenylindole staining) after the addition of 50 μM IPTG.
