Table 1.
The 11 protein-encoding loci analyzed
| Locus | Encoding | Noncoding DNA | Total bp/noncoding bp | Restriction enzyme |
|---|---|---|---|---|
| 1. amdS12 | Acetamidase | 3 introns | 550/≈153 | HinfI |
| 2. pecA12 | Polygalacturonase | 3 introns | 533/≈211 | DdeI |
| 3. omt12 | O-Methyltransferase | 3 introns | 458/≈171 | TaqI |
| 4. niaD12 | Nitrate reductase | 1 intron | 465/≈56 | DpnII |
| 5. benA56 | β-Tubulin | none | 411/0 | DpnII |
| 6. pgkA12 | Phosphoglycerate kinase | 1 intron | 482/≈72 | DpnII |
| 7. facB34 | Acetate regulation | 5′ NT | 469/≈402 | ScaI |
| 8. glaA12 | Glucoamylase | 2 introns | 499/≈92 | DpnII |
| 9. trpC13 | Tryptophan synthesis | 5′ NT | 506/≈486 | MseI |
| 10. cmdA78 | Calmodulin | 5′ NT | 468/≈308 | XbaI |
| 11. gsdA12 | Glucose-6-phosphate dehydrogenase | 3 introns | 623/≈467 | TaqI |
The numbers in each locus name represent the names of the primers used. 5′ NT, noncoding DNA just 5′ to the first amino acid of the gene.