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. 2000 Apr 11;97(8):4297–4302. doi: 10.1073/pnas.97.8.4297

Figure 2.

Figure 2

The mraY4 allele causes a general lysis defect. (A) Lysis profiles from pEmycZ induction in different genetic backgrounds. The allelic state of slyD and mraY in each strain is as follows: slyD+ mraY+ (RY7425) (○), slyD1 mraY+ (RY7426) (□), slyD+ mraY4 (RY7427) (▵), and slyD1 mraY4 (RY7428) (+). The arrow indicates the time of IPTG addition to induce lysis gene expression. (B) The strains are the same as indicated in A except they contain the plasmid pEposmycZ. (C) Same as in A except strains contain the plasmid pE35GFP. (D) Lysis profiles resulting from increased mraY expression. Emyc expression was induced from CAG12095 pBAD30 pEmycZK (○), pMY30 pEmycZK (□), and pMY304 pEmycZK (▵) at time 0. All cultures were grown in the presence of 0.2% arabinose for constitutive expression of the mraY alleles cloned in the pBAD30 vector.