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. 1993 Aug;59(8):2730–2733. doi: 10.1128/aem.59.8.2730-2733.1993

Rapid Mini-Prep Isolation of High-Quality Plasmid DNA from Lactococcus and Lactobacillus spp. †

Daniel J O'Sullivan 1, Todd R Klaenhammer 1,*
PMCID: PMC182348  PMID: 16349028

Abstract

A simple, rapid plasmid mini-prep procedure for lactococci and lactobacilli which gives high yields and can be performed on overnight broth cultures is presented. Large plasmids were isolated from both lactococci and lactobacilli, including a 70-kb plasmid from Lactobacillus acidophilus C7. The purity of the resulting plasmid DNA makes it suitable for subsequent molecular manipulations. The convenience of the technique makes this rapid mini-prep procedure suitable for routine plasmid isolation from lactic acid bacteria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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