Figure 1.

Cloning and sequence analysis of zfBmal cDNAs. (A) Yeast growth is supported on selective medium in two-hybrid assays including both CLOCK and either of the zfBMALs (clones 40–1 and 40–2). (B) DNA sequence identity between the Bmal cDNAs. The hatched bar in 40–1/6 represents the region differing between the two clones. The ORF is denoted by a thicker box. (C) Alignment of human and zebrafish BMALs. Except for their amino terminus, human isoforms a and b are identical. Identical (inverted) and similar (gray background) residues are indicated. Hatched and solid underlined sequences highlight the bHLH domain and the PAS domain repeats, respectively. (D) Phylogenetic analysis of BMAL proteins. The result of 100 bootstrap resamplings is shown. mARNT1, mARNT2, and mCLOCK were used to root the tree (see Materials and Methods for details).