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. 2000 Apr 11;97(8):4339–4344. doi: 10.1073/pnas.97.8.4339

Figure 3.

Figure 3

Rhythmic expression of the Bmal genes in the zebrafish brain, eyes, and pineal gland. (A, C, and D) RPA of the expression of the Bmal transcripts in the brain, eyes, and pineal gland under LD (A and D) or constant darkness (C) conditions. Equivalent amounts of RNA were used in each case, as assayed by ethidium staining and CREB (cAMP responsive binding protein) RPA control. “t” is the negative control with only tRNA. Numbers above each lane correspond to the ZT points at which RNA samples were prepared. (A and D) White/black bars represent the duration of the LD periods. (C) Gray/black bars show the extent of subjective day/night periods in dark-dark (DD) conditions. (B) In situ hybridization of zebrafish brain sections at ZT3 or ZT12 (in LD). For each time point, two successive sections were analyzed with Bmal1 and Bmal2 probes.