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Hsp101 and small Hsps accumulate normally in mutant seedlings after heat stress. Total proteins from 2.5-day-old dark grown seedlings were isolated either before (C) or after treatment at 38°C for 90 min, followed by 2 h at 22°C (H). Proteins were analyzed by Western blotting with either Hsp101 or Hsp17.6 antibodies.
