Trace cued and contextual fear conditioning—galanin-overexpressing transgenic mice: experiment 2. Performance of an independent group of wild-type littermates (WT), and galanin-overexpressing transgenic (GAL-tg) mice in a second trace cued and contextual fear conditioning experiment (A) Freezing behavior during the first 2 min of the training before CS–US presentation (Pre US), and the final 2 min of training following CS–US presentations (Post US). (B) Freezing behavior in the novel context, 24 h after training, during the first 3 min of no CS presentation (Pre Cue), 3 min of CS presentation (Auditory Cue), and 1.5 min following offset of the CS (Post Cue). (C) Freezing behavior 48 h after training, in the same context in which training was carried out on day 1. GAL-tg mice displayed significantly less freezing to the auditory cue in the trace fear conditioning task. Experiment 2 replicated the results of experiment 1, shown in Fig. 6. Data are presented as means ± SEM, (*) P = .011, GAL-tg versus wild type.