Figure 2.

Wortmannin administration improves long-term memory for contextual fear conditioning. Animals were trained in the conditioned emotional response task as outlined in the Materials and Methods section and tested for long-term memory 48 h later. Post-training administration of 8.5 ng/hippocampus wortmannin (n = 7) improved (A) context-specific long-term memory without any effect on (B) cue-specific long-term memory compared to vehicle-infused controls (n = 7). (C) Similar effects on contextual long-term memory were seen following intrahippocampal administration of 5 μg/hippocampus wortmannin (vehicle, n = 7; wortmannin, n = 8). (D) When administered 2 h prior to memory testing, no effect of 8.5 ng/hippocampus wortmannin was observed (vehicle, n = 9; wortmannin, n = 9). Data are presented as the mean ± SEM; * indicates significant (p<0.05) differences between the performance curves.