TABLE 2.

Primers and annealing temperatures used in the study

Gene	Primer	Tm (°C)a	GenBank Accession no.
KpgyrA1	5′-TGCGAGAGAAATTACACC-3′	56	X16817
KpgyrA2	5′-AATATGTTCCATCAGCCC-3′
KpmarR1	5′-CCAGCGACCTGTTTAATGA-3′	56	M96235
KpmarR2	5′-GCGTCATTATTACGTCTGG-3′
KpmarA1	5′-TGCTCAAGAAGGTCCTGCC-3′	58	M96235
KpmarA2	5′-TGCGGCAGCGAATAGTTTC-3′
KpsoxS1	5′-CCCATCAGGATATTATTCA-3′	52	U00734
Kpsox2	5′-AGATGTGATGGCGATAGT-3′
KpacrE1	5′-ATGACGACTCACGCCA-3′	50	M96848
KpacrE2	5′-CGTTTCACCGTCAAATG-3′
KpramA1	5′-GGGTCGCCGATAAGACGC-3′	60	U19581
KpramA2	5′-GCTGGGCGCCATTGAGTAT-3′
Kpramop1	5′-TATCAACGGCTGGCGGCT-3′	58	U19581
Kpramop2	5′-GCAGCGGTTGATGCAGGT-3′
KpacrRNru1b	5′-TGAGTCGCGAATTAAGCTGACAAGCTCTC-3′	58	AJ318073
KpacrRBcl1c	5′-TGAGTGATCAGGTCATGCTATGGTACATA-3′
KpacrA1	5′-ATGAACAAAAACAGAGG-3′	52	AJ318073
KpacrA2	5′-TTTCAACGGCAGTTTTCG-3′

^aT_m, melting temperature.

^bUnderlining indicates the Nru1 site.

^cUnderlining indicates the Bcl1 site.