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. 2003 Sep;47(9):2997–3001. doi: 10.1128/AAC.47.9.2997-3001.2003

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Copyright © 2003, American Society for Microbiology

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FIG. 1. — Scheme showing the construction of plasmids for complementation testing. The primers ( ) for PCR were as follows: primers PAO561-s2 and PAO561-a for the tcp structural region only and primers PAO561-s1 and PAO561-a for the tcp structural gene and the upstream region of orf2. Plasmid pTKM02 carried the tcp structural region in the orientation opposite that of the lac promoter in the pCR2.1 vector. Plasmid pTKM03 carried the tcp and the orf2 in the reverse direction, and pTKM04 carried the tcp structural gene in the forward direction. The tcp gene [ ], orf2 gene [ ], and lac promoter [ ] are shown. Drawings are not to scale. MCS, multiple cloning site.

Inline graphic — Scheme showing the construction of plasmids for complementation testing. The primers ( ) for PCR were as follows: primers PAO561-s2 and PAO561-a for the tcp structural region only and primers PAO561-s1 and PAO561-a for the tcp structural gene and the upstream region of orf2. Plasmid pTKM02 carried the tcp structural region in the orientation opposite that of the lac promoter in the pCR2.1 vector. Plasmid pTKM03 carried the tcp and the orf2 in the reverse direction, and pTKM04 carried the tcp structural gene in the forward direction. The tcp gene [ ], orf2 gene [ ], and lac promoter [ ] are shown. Drawings are not to scale. MCS, multiple cloning site.